3.6B · BIOLOGY ONLY

3.6B: Transpiration

Transpiration as evaporation from plant surfaces and water-vapour loss through stomata.

LEARNING GOALS

By the end of this lesson

  • Define transpiration accurately.
  • Trace water from xylem to atmosphere.
  • Explain benefits and costs of water loss.

BIG QUESTION

Why does a plant lose most of the water it absorbs?

Evaporation from moist mesophyll surfaces supports gas exchange but creates a continuous water demand.

Evaporation begins inside the leaf

Water moves from xylem into mesophyll cells and moistens their cell walls. It then evaporates into the leaf air spaces.

Water vapour diffuses through open stomata because the outside air is usually less humid.

Transpiration sustains a stream

is evaporation of water from plant surfaces, especially leaves. The loss lowers leaf water potential and pulls replacement water through xylem.

This also carries mineral ions and can cool leaves.

Stomata balance carbon gain and water loss

Open stomata allow carbon dioxide to enter for photosynthesis but also permit water vapour to leave.

Guard cells regulate stomatal aperture. During water stress, closure conserves water but can limit photosynthesis.

Stages of leaf water loss

Precise answers distinguish movement in liquid and gas phases.

Transpiration pathway
StageState/processLocation
Xylem to mesophyllLiquid water movement.Leaf veins and cells.
Cell wall to air spaceEvaporation.Moist mesophyll surface.
Air space to atmosphereDiffusion of water vapour.Through stomata.
Replacement from rootsMass flow under tension.Xylem.
KEY TERMS

Language that earns precision

transpiration
Evaporation of water from plant surfaces.
stoma
Adjustable pore in the epidermis for gas exchange.
guard cell
Cell controlling stomatal opening.
transpiration stream
Continuous movement of water from roots through xylem to leaves.

Common misconceptions

Claim: Transpiration is water moving up xylem.

Correction: It is evaporation from plant surfaces; xylem flow replaces lost water.

Claim: Water leaves as liquid droplets through stomata.

Correction: It normally leaves as water vapour.

Claim: All water loss is useless.

Correction: It accompanies gas exchange, moves minerals and can cool leaves.

EXAM LANGUAGE

Match the answer to the command word

Define

Use evaporation from the surface; do not define it as water uptake.

Trace

Write xylem → mesophyll wall → air space → stoma → atmosphere.

CHECK YOUR UNDERSTANDING

Recall, apply, then justify

01Explain5 marks

Explain the pathway of water through a leaf during transpiration.

Hint

Begin at xylem and name the state change.

Suggested marking points
  • Water moves from xylem into mesophyll cells/cell walls.
  • It evaporates from moist cell walls.
  • Water vapour enters leaf air spaces.
  • It diffuses down a concentration gradient.
  • It leaves through stomata.
02Explain4 marks

Explain one benefit and one cost of stomatal opening.

Hint

Carbon dioxide and water.

Suggested marking points
  • Open stomata allow carbon dioxide to diffuse in.
  • This supports photosynthesis.
  • They also allow water vapour to diffuse out.
  • Excess loss can reduce turgor/cause wilting or stomatal closure.
STRETCH AND CHALLENGE

Explain how transpiration can cool a sunlit leaf yet also reduce photosynthesis during drought.

Planning hint

Evaporation uses energy; drought changes stomata.

What a strong design includes
  • Evaporation requires latent heat energy.
  • Energy is removed from leaf tissue, lowering temperature.
  • Drought lowers plant water potential/turgor.
  • Guard cells close stomata to reduce water loss.
  • Less carbon dioxide enters.
  • Carbon dioxide can then limit photosynthesis.
END OF THE LESSON

What next?

Choose an activity, or move on to the next lesson.

Practise 3.6B: 15 recall questions · 14 exam questions