2(d) MOVEMENT OF SUBSTANCES INTO AND OUT OF CELLS · RECALL

2.17 Diffusion and osmosis investigations

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012.17Pearson Edexcel focus

How do you calculate percentage change in mass?

Reveal answer

(final mass − initial mass) ÷ initial mass × 100.

Key words: percentage change

022.17Pearson Edexcel focus

How do you estimate cell sap concentration from potato results?

Reveal answer

Plot % change in mass against concentration; read off where the line crosses zero.

Key words: crosses zero

032.17Pearson Edexcel focus

Give two control variables for a potato osmosis investigation.

Reveal answer

Same size of cylinder (same cork borer and length), same time, same temperature, same volume of solution, same potato.

Key words: same size · same time

042.17Pearson Edexcel focus

Why blot potato cylinders before weighing?

Reveal answer

To remove surface liquid so only water taken in or lost by the cells is measured.

Key words: blot

052.17Lesson

What is meant by the term percentage change?

Reveal answer

(final value − initial value) ÷ initial value × 100.

Key words: percentage change

062.17Lesson

What is meant by the term isotonic?

Reveal answer

Having equal effective water potential across a membrane, causing no net water movement.

Key words: isotonic

072.17Lesson

What is meant by the term x-intercept?

Reveal answer

The point where a plotted relationship crosses the horizontal axis.

Key words: x-intercept

082.17Lesson

What is meant by the term model?

Reveal answer

A simplified representation used to investigate or explain selected features.

Key words: model

092.17Lesson

What is meant by the term biological variation?

Reveal answer

Natural differences among living samples.

Key words: biological variation

102.17Lesson

Two practical systems, two kinds of evidence: for System, give the indicator agar and the potato tissue.

Reveal answer

Indicator agar: Non-living gel. Potato tissue: Living multicellular tissue.

Key words: System

112.17Lesson

Two practical systems, two kinds of evidence: for Process emphasised, give the indicator agar and the potato tissue.

Reveal answer

Indicator agar: Acid diffusion through a volume. Potato tissue: Osmosis across cell membranes.

Key words: Process emphasised

122.17Lesson

Two practical systems, two kinds of evidence: for Independent variable, give the indicator agar and the potato tissue.

Reveal answer

Indicator agar: Cube size/SA:V or another defined diffusion factor. Potato tissue: External sucrose concentration.

Key words: Independent variable

132.17Lesson

Two practical systems, two kinds of evidence: for Dependent variable, give the indicator agar and the potato tissue.

Reveal answer

Indicator agar: Penetration distance or percentage decolourised. Potato tissue: Percentage mass change.

Key words: Dependent variable

142.17Lesson

Two practical systems, two kinds of evidence: for Major limitation, give the indicator agar and the potato tissue.

Reveal answer

Indicator agar: No cell membrane or metabolism. Potato tissue: Biological variation, cut damage and blotting inconsistency.

Key words: Major limitation

152.17Lesson

True or false, and why? “Acid moves by osmosis into agar.”

Reveal answer

False. The acid particles diffuse; osmosis specifically describes water through a partially permeable membrane.

162.17Lesson

True or false, and why? “A potato cylinder with zero mass change has no water movement.”

Reveal answer

False. Water moves both ways at equal rates, producing no net change.

172.17Lesson

True or false, and why? “A larger potato piece can be compared using mass change alone.”

Reveal answer

False. Percentage mass change standardises for different initial masses.

182.17Lesson

True or false, and why? “Agar proves exactly how a cell behaves.”

Reveal answer

False. It lacks a cell membrane, metabolism and active transport, so it is a limited diffusion model.

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