2(c) BIOLOGICAL MOLECULES · EXAM QUESTIONS

2.12 Temperature and enzyme activity

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  1. 01CalculateCalculation2 marks

    2.12 Temperature and enzyme activity

    Exam question

    Mark points shown after marking

    A student found that amylase took 125 s to digest starch at 35 °C. Calculate the rate of reaction using rate = 1 ÷ time. Give your answer to 3 decimal places.

    (2)
    s⁻¹

    Total for this question: 2 marks

  2. 02CalculateCalculation2 marks

    2.12 Temperature and enzyme activity

    Exam question

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    At 25 °C, a protease took 320 s to clear a photographic film strip. Calculate the rate as 1 ÷ time to 4 decimal places.

    (2)
    s⁻¹

    Total for this question: 2 marks

  3. 03DescribeShort answer4 marks

    2.12 Temperature and enzyme activity

    Exam question

    Mark points shown after marking

    Describe how you could investigate the effect of temperature on the activity of amylase.

    (4)

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    Total for this question: 4 marks

  4. 04ExplainShort answer2 marks

    2.12 Temperature and enzyme activity

    Exam question

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    Explain why the amylase and starch solutions are left in the water bath for a few minutes before being mixed.

    (2)

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    Total for this question: 2 marks

  5. 05ExplainShort answer2 marks

    2.12 Temperature and enzyme activity

    Exam question

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    Explain why a buffer solution is added in an investigation into the effect of temperature on an enzyme.

    (2)

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    Total for this question: 2 marks

  6. 06SuggestShort answer2 marks

    2.12 Temperature and enzyme activity

    Exam question

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    Suggest how a student could improve the reliability of results in an enzyme temperature investigation.

    (2)

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    Total for this question: 2 marks

  7. 07ExplainShort answer2 marks

    2.12 Temperature and enzyme activity

    Exam question

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    Explain why iodine solution is used in the amylase investigation.

    (2)

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    Total for this question: 2 marks

  8. 08DescribeShort answer2 marks

    2.12 Temperature and enzyme activity

    Exam question

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    Describe how to keep the temperature constant in an enzyme investigation.

    (2)

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    Total for this question: 2 marks

  9. 09IdentifyShort answer2 marks

    2.12 Temperature and enzyme activity

    Exam question

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    Identify the independent and the dependent variable in an investigation into the effect of temperature on how long lipase takes to digest milk fat.

    (2)

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    Total for this question: 2 marks

  10. Plan it with CORMMSS: Design an investigation (Logic Lab).

    10DesignDesign an investigation6 marks

    2.12 Temperature and enzyme activity

    Exam question

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    Design an investigation to find out how temperature affects the rate at which the enzyme catalase breaks down hydrogen peroxide. Write your answer in full sentences.

    (6)

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    Total for this question: 6 marks

  11. 11ExplainShort answer2 marks

    2.12 Temperature and enzyme activity

    Exam question

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    Explain why the same volume of iodine is placed in each well of the spotting tile.

    (2)

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    Total for this question: 2 marks

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