3.35

3.35: Auxin and phototropism in stems

Auxin redistribution and unequal cell elongation in the phototropic response of stems.

LEARNING GOALS

By the end of this lesson

  • Describe auxin production and redistribution.
  • Explain unequal shoot elongation.
  • Interpret classic phototropism experiments.

BIG QUESTION

How can light on one side make a shoot bend toward it?

Directional light changes auxin distribution; greater auxin action on the shaded side promotes elongation and curvature.

The shoot tip detects directional light

Light-sensitive processes near the shoot tip detect a difference between illuminated and shaded sides. is produced mainly in young shoot tissues and transported away from the tip.

Modern evidence supports lateral redistribution and transport; avoid the oversimplified claim that light merely destroys auxin.

Auxin action becomes greater on the shaded side

Under one-sided light, more auxin is transported to or retained on the shaded side of a shoot. At shoot concentrations, auxin promotes cell elongation.

Cells on the shaded side lengthen more than illuminated-side cells, so the shoot curves toward light.

Experiments separate detection and growth

Removing the tip or covering it with an opaque cap prevents normal phototropic curvature because light detection/auxin signalling is disrupted. A transparent cap usually permits the response.

Covering only the lower shoot does not prevent tip detection, showing that stimulus detection and elongation occur in different regions.

Classic phototropism treatments

Predictions should identify what each manipulation blocks.

Evidence for auxin-controlled phototropism
TreatmentExpected curvatureInference
Intact tip + one-sided lightToward light.Normal detection and signalling.
Tip removedLittle/no curvature.Tip is needed.
Opaque cap on tipLittle/no curvature.Tip must detect light.
Transparent capCurvature occurs.Covering alone is not the cause.
Opaque sleeve below tipCurvature can occur.Light detection is mainly at tip.
KEY TERMS

Language that earns precision

auxin
Plant growth regulator that influences cell elongation and other processes.
differential growth
Different growth rates on opposite sides of an organ.
phototropism
Directional growth response to light.
opaque
Not allowing light to pass through.

Common misconceptions

Claim: Auxin makes the lit side grow faster in a shoot.

Correction: Greater auxin action on the shaded side promotes more elongation there.

Claim: Auxin moves toward light.

Correction: It is redistributed toward the shaded side under directional light.

Claim: Light simply destroys all auxin on one side.

Correction: Phototropism involves regulated transport and redistribution, not only destruction.

EXAM LANGUAGE

Match the answer to the command word

Explain

Light detected at tip → auxin redistributed → shaded side elongates more → shoot bends toward light.

Evaluate

State what the opaque cap and the transparent cap (a control experiment) each test.

CHECK YOUR UNDERSTANDING

Recall, apply, then justify

01Explain5 marks

Explain positive phototropism in a shoot using auxin.

Hint

Detection, distribution, elongation, bend.

Suggested marking points
  • Directional light is detected near the shoot tip.
  • Auxin is redistributed toward the shaded side.
  • Auxin promotes shoot-cell elongation.
  • Shaded-side cells elongate more than lit-side cells.
  • Unequal growth bends the shoot toward light.
02Explain3 marks

Explain why an opaque cap prevents curvature but a transparent cap does not.

Hint

Use the transparent cap as a control experiment for covering, separate from light detection.

Suggested marking points
  • An opaque cap prevents directional light reaching the tip.
  • Light detection/auxin redistribution is disrupted.
  • A transparent cap allows light through, so detection and curvature can continue.
STRETCH AND CHALLENGE

Design an investigation distinguishing whether light is detected at the tip or elongation zone.

Planning hint

Use matched cap and sleeve treatments.

What a strong design includes
  • Use seedlings of the same species, age and initial length.
  • Expose all groups to the same one-sided light, temperature and water conditions.
  • Include an intact control group.
  • Place opaque caps on tips in one group.
  • Place transparent caps on tips as a covering control experiment.
  • Place opaque sleeves below tips on another group.
  • Measure curvature angle after a fixed time.
  • Use replicates and compare mean curvature between groups.
END OF THE LESSON

What next?

Choose an activity, or move on to the next lesson.

Practise 3.35: 15 recall questions · 16 exam questions