6(a) FOOD PRODUCTION · RECALL

6.7 Lactobacillus and yoghurt

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016.7Pearson Edexcel focus

Steps to make yoghurt?

Reveal answer

Pasteurise → cool to 40 °C → add Lactobacillus → incubate → lactic acid thickens.

Key words: Lactobacillus

026.7Pearson Edexcel focus

What does Lactobacillus make from lactose?

Reveal answer

Lactic acid.

Key words: lactic

036.7Pearson Edexcel focus

Why does yoghurt thicken?

Reveal answer

Lactic acid → low pH → milk proteins coagulate.

Key words: coagulate

046.7Pearson Edexcel focus

Why pasteurise milk first?

Reveal answer

Kill unwanted bacteria.

Key words: bacteria

056.7Lesson

What is meant by the term Lactobacillus?

Reveal answer

A genus of bacteria that ferment sugars to lactic acid.

Key words: Lactobacillus

066.7Lesson

What is meant by the term pasteurisation?

Reveal answer

Heat treatment that kills most microorganisms in a food.

Key words: pasteurisation

076.7Lesson

What is meant by the term incubation?

Reveal answer

Keeping a culture at a controlled temperature for growth.

Key words: incubation

086.7Lesson

Steps in yoghurt production: for Pasteurisation, give the conditions and the reason.

Reveal answer

Conditions: Heat milk, e.g. 85–95 °C. Reason: Kill unwanted microorganisms.

Key words: Pasteurisation

096.7Lesson

Steps in yoghurt production: for Cooling, give the conditions and the reason.

Reveal answer

Conditions: To about 40–45 °C. Reason: So added bacteria are not killed.

Key words: Cooling

106.7Lesson

Steps in yoghurt production: for Add starter culture, give the conditions and the reason.

Reveal answer

Conditions: Lactobacillus. Reason: Ferments lactose.

Key words: Add starter culture

116.7Lesson

Steps in yoghurt production: for Incubation, give the conditions and the reason.

Reveal answer

Conditions: About 40–45 °C for hours. Reason: Optimum for bacterial enzymes; lactic acid builds up.

Key words: Incubation

126.7Lesson

Steps in yoghurt production: for Cooling and storage, give the conditions and the reason.

Reveal answer

Conditions: Refrigeration. Reason: Slows further fermentation and spoilage.

Key words: Cooling and storage

136.7Lesson

True or false, and why? “Lactobacillus produces ethanol like yeast.”

Reveal answer

False. It produces lactic acid.

146.7Lesson

True or false, and why? “Pasteurisation sterilises milk completely.”

Reveal answer

False. It kills most, not all, microorganisms.

156.7Lesson

True or false, and why? “Yoghurt thickens because bacteria add protein.”

Reveal answer

False. Lactic acid lowers pH, causing existing milk proteins to coagulate.

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