Dolly cloning steps?
Reveal answer
Enucleate egg → insert body cell nucleus → shock → embryo → surrogate.
Key words: surrogate
6(d) CLONING · RECALL
6.19B Cloning mammals
15 recall questions · Biology only (B): not in Science (Double Award). Answer from memory, reveal the answer, then rate yourself. Ratings stay on this device.
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Dolly was identical to…?
Reveal answer
The body cell donor.
Key words: donor
Why give an electric shock?
Reveal answer
Stimulate cell division.
Key words: division
Name of the cloning method?
Reveal answer
Nuclear transfer.
Key words: nuclear
Disadvantages of cloning?
Reveal answer
Low success, less variation, health problems.
Key words: variation
Role of surrogate mother?
Reveal answer
Carries the embryo.
Key words: embryo
What is meant by the term enucleated?
Reveal answer
Having had the nucleus removed.
Key words: enucleated
What is meant by the term surrogate mother?
Reveal answer
A female who carries an embryo that is not genetically hers.
Key words: surrogate mother
What is meant by the term embryo?
Reveal answer
An early stage of development formed by mitosis.
Key words: embryo
Three sheep, one clone, Finn Dorset (nucleus donor). What it provided? Is Dolly genetically identical to it??
Reveal answer
What it provided: Diploid nucleus from an udder cell. Is Dolly genetically identical to it?: Yes.
Key words: Finn Dorset (nucleus donor)
Three sheep, one clone, Scottish Blackface (egg donor). What it provided? Is Dolly genetically identical to it??
Reveal answer
What it provided: Enucleated egg cell. Is Dolly genetically identical to it?: No (nuclear DNA removed).
Key words: Scottish Blackface (egg donor)
Three sheep, one clone, Scottish Blackface (surrogate). What it provided? Is Dolly genetically identical to it??
Reveal answer
What it provided: Uterus for development. Is Dolly genetically identical to it?: No.
Key words: Scottish Blackface (surrogate)
True or false, and why? “Dolly is identical to the surrogate who gave birth to her.”
Reveal answer
False. She is genetically identical to the nucleus donor.
True or false, and why? “A sperm cell fertilises the egg in cloning.”
Reveal answer
False. No fertilisation occurs; the diploid nucleus is inserted.
True or false, and why? “The egg’s nucleus is used.”
Reveal answer
False. The egg nucleus is removed; the body cell nucleus is used.