PAPER 2 · QUESTION 1 STYLE · LINEAR PAPER 2

Last chance for the northern white rhino

350 words over 33 numbered lines, then 7 parts worth 14 marks (Linear Paper 2 (4BI1/2B) style, 14–18 marks). Read the article, answer each part, then open its mark scheme and set your mark.

4BI1/2B14 marksabout 17 minutes

Article

Illustrative article written for Scholar Lab, not a Pearson text. The biology is real; the studies, people and figures are invented or rounded for practice.

Last chance for the northern white rhino

In a guarded enclosure in Kenya live the last two northern white

rhinos on Earth. Both are female, and neither can carry a pregnancy.

The last male died in 2018. For most species, that would be the end of

the story. For this one, scientists are trying to use reproductive

technology to bring it back.

Before the last males died, their sperm was collected and frozen in

liquid nitrogen. Every few months, vets give one of the females an

anaesthetic and collect egg cells from her ovaries. The eggs are flown

to a laboratory in Europe, where each egg is injected with a single

sperm cell. If fertilisation happens, the zygote divides to form an

embryo, which is frozen until it can be used.

In an illustrative count, 220 eggs had been collected by the end of

one year and 33 embryos had been produced. Because neither female can

carry a pregnancy, the embryos will be placed in the uterus of a

female southern white rhino, a closely related subspecies.

There is another problem. All the embryos come from just two females

and a few males, so the new population would have very little genetic

variation. Scientists have therefore stored skin cells from twelve

northern white rhinos that died in zoos over the past forty years.

They hope to use these cells to make new gametes in the laboratory, or

to produce cloned rhinos.

Cloning a large mammal is difficult. Sheep, cattle and horses have

been cloned successfully, but most cloned embryos fail to develop. A

cloned rhino would also be genetically identical to the animal that

provided the skin cell, so cloning alone cannot add new variation. It

can, however, bring back alleles that were lost when the zoo animals

died.

Not everyone supports the project, which has cost many millions of

dollars. Critics say that the money would protect more animals if it

were spent on stopping poaching and protecting the habitats of rhinos

that still survive in the wild. Supporters reply that the techniques

they develop could also help to save other endangered mammals in the

future.

Read the article. Use the information in the article and your own knowledge to answer the questions. The lines in brackets show where to look; select them to highlight the text.

Use the information in the article and your own knowledge to answer the questions. The lines in brackets show you where to look; select them to highlight the text.

(a)

State what is meant by the term fertilisation.

(1)
(b)

Which describes the number of chromosomes in a cell of the embryo compared with the number in an egg cell?

(1)
Choose one answer for part b
(c)

Calculate the percentage of collected eggs that produced embryos.

(2)
(d)

Explain why the embryos must be placed in the uterus of a female southern white rhino.

(2)
(e)

Describe how a skin cell from a northern white rhino could be used to produce a cloned rhino.

(3)
(f)

Explain why a population with very little genetic variation is at risk.

(3)
(g)

Suggest why the critics think the money should be spent on protecting habitats and stopping poaching.

(2)

Total for this question: 14 marks

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