6(c) GENETIC MODIFICATION (GENETIC ENGINEERING) · EXAM QUESTIONS

6.12 Restriction enzymes and ligase

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  1. 01DescribeShort answer3 marks

    6.12 Restriction enzymes and ligase

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    Describe the roles of restriction enzymes and ligase in genetic modification.

    (3)

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    Total for this question: 3 marks

  2. 02WhichMultiple choice1 mark

    6.12 Restriction enzymes and ligase

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    What do restriction enzymes produce?

    (1)
    Choose one answer

    Total for this question: 1 mark

  3. 03ExplainShort answer2 marks

    6.12 Restriction enzymes and ligase

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    Explain why the same restriction enzyme is used to cut the plasmid and the human DNA.

    (2)

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    Total for this question: 2 marks

  4. 04ExplainShort answer2 marks

    6.12 Restriction enzymes and ligase

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    Explain why sticky ends are useful.

    (2)

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    Total for this question: 2 marks

  5. 05StateShort answer1 mark

    6.12 Restriction enzymes and ligase

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    State the enzyme that joins DNA fragments.

    (1)

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    Total for this question: 1 mark

  6. 06ExplainShort answer2 marks

    6.12 Restriction enzymes and ligase

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    Explain why restriction enzymes only cut at certain places.

    (2)

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    Total for this question: 2 marks

  7. 07IdentifyShort answer1 mark

    6.12 Restriction enzymes and ligase

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    Identify the term for DNA that contains genes from two different organisms.

    (1)

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    Total for this question: 1 mark

  8. 08SuggestShort answer2 marks

    6.12 Restriction enzymes and ligase

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    Suggest why bacteria naturally produce restriction enzymes.

    (2)

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    Total for this question: 2 marks

  9. 09DescribeShort answer2 marks

    6.12 Restriction enzymes and ligase

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    Describe what is meant by a sticky end.

    (2)

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    Total for this question: 2 marks

  10. 10ExplainShort answer2 marks

    6.12 Restriction enzymes and ligase

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    Explain why ligase is needed even though sticky ends pair.

    (2)

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    Total for this question: 2 marks

  11. 11GiveShort answer1 mark

    6.12 Restriction enzymes and ligase

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    Give the type of DNA molecule often cut open to carry a human gene into bacteria.

    (1)

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    Total for this question: 1 mark

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